WVU Research Corporation Biology Technician in Morgantown, West Virginia

Description Purpose The purpose of this position is to work with the Principal Investigator (PI) on breast cancer research involving molecular biology, cell culture, and model animal (mice) approaches.

This position performs various support duties, and responds to inquiries and resolves matters of relatively low complexity. Responsibilities require some basic decision making and resourcefulness within available guides and precedents. Work effort impacts program and project image with nominal liabilities.

Duties & Responsibilities Responsibilities are functional in nature, and performed under direct supervision. Specific tasks include, but are not limited to: 1) Understanding and utilizing lab protocols from current literature. 2) Independently perform cell culture experiments after consulting with the PI. 3) Preparing cell and tissue extracts and analyzing gel electrophoresis and western blotting or performing immunoprecipitation/affinity precipitation followed by gel electrophoresis and western blotting. 4) Bacterial culture, DNA purification, protein expression and purification. 5) PCR genotyping of model mice. 6) Data recording. 7) Laboratory oversight.

Supervisory/Lead Role Reports to the Principle Investigator. This position will normally receive or follow specific instructions for routine work. Supervision of others is not required, however, sharing of knowledge with others and team participation is expected in the role. Physical Demands Activities require manual skill to perform accurate micropipetting of samples, mixing of small volumes of DNA/RNA, standing sitting by the bench, lifting of fluid-containing carboys, bending, carrying lightweight objects (up to 40 lbs), and transporting mice from laboratory to animal facility, traversing stairs or using elevators. Good hand-eye coordination, precision of movements, and attention to detail is required.

Work Environment Work will be performed in a standard laboratory environment including using computers and laboratory equipment with electrical connections. Some work will be performed in the cold room and some laboratory chemicals have an odor or toxic fumes. Some work will be performed in the dark room either under dim red light or in the complete darkness using night vision equipment. There will be exposure to murine tissues, laboratory animals and radiation, however, protective measures will be provided.

The following items have been determined as possible job exposures of this position requiring WVU EHS medical monitoring: 1) Animal Contact- direct exposure to vertebrate animals, animal tissues or research with body fluids or wastes 2) Contacting Hazardous Chemicals- daily or weekly, using strong acids or caustics, fuel or tar products or cancer-causing chemicals 3) Scientific Research- research with nanoparticles, lasers, anesthetic, fuels or mining, aquatic or diving

Requirements Requisite Qualifications Education and/or Experience 1) Bachelor’s degree in biology, biomedical laboratory technology, or a related field And 2) 6 months – 2 years of related experience in a biomedical setting Or 3) An equivalent combination of education and/or experience.

Skills, Knowledge, Abilities 1) Experience in the following areas: a. DNA & RNA isolation from various tissues, genotyping samples by PCR / restriction digestion / sequence analysis, b. DNA cloning, maintenance of tissue culture cells and working with laboratory animals. 2) Skilled in using Microsoft Office and statistical software, such as Graph Pad. 3) Ability to modify and optimize research protocols as needed for the successful completion of the specific experiment. 4) Knowledge and laboratory experience in general genetics/molecular biology/neurobiological techniques. 5) Ability to maintain tissue culture cells and work with laboratory animals 6) Ability to modify and optimize research protocols as needed for successful completion of the specific experiment. 7) Knowledge of laboratory environment and appropriate procedures. 8) Skilled at using computers and applicable programs/applications. 9) Skilled at assessing chemical concentrations/amounts of various chemical components in solutions. 10) Knowledge of plant and animal organisms, their tissues, cells, functions, interdependencies and interactions with each other and the environment. 11) Knowledge of the chemical composition, structure, and properties of substances and of the chemical processes and transformations that they undergo. This includes uses of chemicals, and their interactions, danger signs, production techniques, and disposal methods. 12) Skilled at using scientific rules and methods to solve problems. 13) Skilled at using related laboratory equipment. 14) Ability to teach, train, oversee and assist other staff, postdoctoral fellows and graduate students. 15) Good organizational skills and self-motivation. 16) Willingness and ability to learn new protocols in a rapid manner. 17) Ability to get along with others in a group setting.

Behavioral Competencies 1) Technical skills – Strives to continuously build knowledge and skills; pursues training and development opportunities; assesses strengths, weaknesses, and shares expertise with others. 2) Problem solving – Gathers and analyzes information, identifies problems, develops alternatives, uses reason even when dealing with emotional topics and works well in groups and resolves matters timely. 3) Internal & External Customer Service – Manages difficult or emotional matters with objectivity and openness; responds promptly to needs and requests for service and assistance; meets commitments and dates, notifies others if change occurs; provides, solicits and applies feedback; and builds goodwill through balanced contributions, positive approaches, and demonstrating respect and support of others. 4) Communications – Demonstrates group presentation skills, participates in meetings, listens and gets clarification, is responsive and speaks clearly and persuasively in positive and negative situations. Writes and edits clearly and informatively, varies style to meet needs, presents numerical data effectively, and is able to read and interpret written information. 5) Organizational Support – Aligns work and develops strategies to meet organizational goals; seeks to improve and promote quality; demonstrates accuracy and thoroughness; and upholds organizational expectations and values. 6) Self-Management – Responds to management direction; resourcefully adapts to changes in work environment and circumstances; responds well under pressure and manages competing demands; takes calculated risks, asks for and offers help when needed; exercises discretion and sound judgment; is tactful and responsible for own actions. Adheres to work schedule and complies with leave management responsibilities.

Requisition Number: 16-0136

Post Date: 9/16/2016

Title: Biology Technician

Position Type: Full Time

Work Hours: 40 hpw

City: Morgantown

State: WV